Crop Management Impacts the Soybean (Glycine max) Microbiome

Soybean [Glycine max (L.) Merr].

In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wound...

Symbiosis-stimulated chitinase isoenzymes of soybean (Glycine max (L.) Merr.)

and to be colonized by arbuscular mycorrhiza (AM) fungi (Harrison, 1997). Often, nodule formation by a Isoforms of endochitinase in soybean were studied in given bacterial strain is restricted to only a few host plant relation to root symbiosis. Five selected cultivars difgenera, and varieties of a given legume species may differ fering in their nodulation potential were inoculated in nodule fo...

Changes in RNA Splicing in Developing Soybean (Glycine max) Embryos

Developing soybean seeds accumulate oils, proteins, and carbohydrates that are used as oxidizable substrates providing metabolic precursors and energy during seed germination. The accumulation of these storage compounds in developing seeds is highly regulated at multiple levels, including at transcriptional and post-transcriptional regulation. RNA sequencing was used to provide comprehensive in...

Malonyl - Isofiayone Glycosides in Soybean ( Glycine max MERR - . )

Purification and Characterization of Hydroperoxidelyase from soybean (Glycine max)

A fatty acid hydroperoxidelyase (HPL) enzyme from soybean (Glycine max) seeds was purified by ammonium sulphate precipitation followed by ion exchange chromatography on DEAE-Cellulose and DEAE-Sephacel. HPL showed specific activity of 161U/mg with fold purification of 16.4 and enzyme yield was found to be 9.06%. The purified enzyme on SDSPAGE showed a prominent band of ~53KDa. Kinetic studies w...